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  1. Protocol Documents for Processing and Tracking Samples for CCE

    30 Sep 2008 | Notes | Contributor(s): Kyle Nicholas Kurasz, Ann Christine Catlin, Jenifer L. Miller

    Blood samples will be obtained from patients undergoing colonoscopies at the Indiana University Medical Center. Once colonoscopy results are read, samples will be labeled as normal, polyp, or cancer samples. Samples will be processed according to standard methodology and distributed to sample...

  2. API-4000 Instrument Setup : Lipidomics for Cancer Care Engineering

    14 Oct 2008 | Notes | Contributor(s): Zhenwen Zhao, HUI CAI

    This document describes the setup for the API-4000 mass spectrometry instrument in preparation for lipidomics analysis of the plasma processed from the patient whole blood.

  3. Sample Preparation Protocol: Lipidomics for Cancer Care Engineering

    14 Oct 2008 | Notes | Contributor(s): HUI CAI, Zhenwen Zhao

    This document describes the preparation of the plasma sample for lipidomics analysis using the API-4000 mass spectrometer. The plasma was processed from the patient whole blood at the IUSCC clinic immediately following sample acquisition.

  4. Call for Chapters

    21 Jan 2009 | Notes | Contributor(s): Michael Burdick

    This call for chapters seeks foundation-building research on e-research collaboration issues in the Arts and Humanity, Social and Behavioral Sciences, Life Sciences, Engineering, Medicine and Business disciplines.E-Research Collaboration can be defined as the use of information communication...

  5. Sample Preparation : Metabolomics Analysis for Cancer Care Engineering

    13 Feb 2009 | Notes | Contributor(s): Daniel Raftery, Ann Christine Catlin

    This document describes the preparation of serum samples in the Raftery Laboratory for metabolomics GCxCG-MS analysis using the LECO GC-TOF mass spectrometer.Serum samples are processed from patient whole blood at the IUSCC clinic immediately after sample acquisition, and then transferred to the...

  6. LECO GC-TOF Instrument Setup: Metabolomics for Cancer Care Engineering

    13 Feb 2009 | Notes | Contributor(s): Daniel Raftery

    This document describes the setup for the LECO GC-TOF mass spectrometry instrument in preparation for metabolomics GCxGC-MS analysis of serum samples processed from patient whole blood at the IUSCC clinic and transferred to the Raftery Metabolomics Analysis Laboratory. See also Metabolics GCxGC...

  7. Global Proteomics Sample Preparation Protocol

    13 Feb 2009 | Notes | Contributor(s): Catherine Riley

    The urea-based trypsin digestion sample preparation protocol is used for the plasma samples acquired through the Cancer Care Engineering Colorectal Cancer OMIC Study.Prepared samples are analyzed using the XCT PLUS ESI ion trap located in the Bindley Bioscience Center at Purdue University.

  8. XCT PLUS Instrument Setup

    13 Feb 2009 | Notes

    The document describes the instrument setup for the XCT PLUS ESI ion trap located in the Bindley Biosciences Center at Purdue University. Laboratory analysis for global proteomics research is carried out by Jiri Adamec and Paige Riley. The sample preparation protocol describes the metholodology...

  9. XCT PLUS: Agilent 1100 Series HPLC-Chip/MS System

    14 Feb 2009 | Notes

    Nanospray LC/MS is well established as state-of-the-art technology because of its high sensitivity and lowsample consumption. It is most often used for applications with limited sample amounts or when there is a need for analysis of trace level components in complex mixtures. Although nanospray...

  10. Global Proteomics Workflows

    14 Feb 2009 | Notes

    These slides diagram the global proteomics workflows at Bindley Bioscience Center, Purdue University.Catherine Paige Riley, Jiri Adamed, Charles BuckBindley Bioscience CenterPurdue UniversityCatherine P. Riley, Erik S. Gough, Jing He, Shrinivas S. Jandhyala, Brad Kennedy, Seza Orcun, Mourad...