Significance Testing of Normalized LC-MS Data

By Ann Christine Catlin

Purdue University

Several statistical significance tests are employed to identify peptide or metabolite peaks that either make significant contributions to the molecular profile of a sample or distinguish a group of samples from others.

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Version 2.2 - published on 19 Sep 2011

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Abstract

The Significance Test Tool uses several statistical significance test methods to identify data elements that either contribute to the proteomic profile of a sample or that distinguish groups of samples. Some peaks may be present across sample groups but with differing intensity between the groups. The quantitative difference identifies the case where a peak is present in most (or all) samples, but with different intensities from group to group.

Methods implemented in the tool include:

  • Two-tailed t-test and
  • Mann-Whitney tests.



Input for the Significance Test Tool includes:

  • the normalized data file generated from the Normalization Tool for a collection of LC-MS datasets aligned through the Peak Alignmend Tool.
  • input parameters to provide information about the number of groups and group sizes in the normalized dataset.



Cite this work

Researchers should cite this work as follows:

  • Ann Christine Catlin (2011), "Significance Testing of Normalized LC-MS Data," http://ccehub.org/resources/significance.

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