CRC Sample Processing for Cancer Care Engineering

30 Sep. 2008 | Notes | Contributor(s): Julie Nagel, Ann Christine Catlin

Blood samples will be obtained from patients undergoing colonoscopies at the Indiana University Medical Center. Blood samples will also be obtained from spouses or household companions (non-cancer control samples). Once colonoscopy results are read, samples will be labeled as normal, polyp, or …

API-4000 Instrument Setup : Lipidomics for Cancer Care Engineering

14 Oct. 2008 | Notes | Contributor(s): Zhenwen Zhao

This document describes the setup for the API-4000 mass spectrometry instrument in preparation for lipidomics analysis of the plasma processed from the patient whole blood.

Sample Preparation Protocol: Lipidomics for Cancer Care Engineering

14 Oct. 2008 | Notes | Contributor(s): Zhenwen Zhao

This document describes the preparation of the plasma sample for lipidomics analysis using the API-4000 mass spectrometer. The plasma was processed from the patient whole blood at the IUSCC clinic immediately following sample acquisition.

Call for Chapters

21 Jan. 2009 | Notes | Contributor(s): Michael Burdick

This call for chapters seeks foundation-building research on e-research collaboration issues in the Arts and Humanity, Social and Behavioral Sciences, Life Sciences, Engineering, Medicine and Business disciplines. E-Research Collaboration can be defined as the use of information communication …

Sample Preparation : Metabolomics Analysis for Cancer Care Engineering

13 Feb. 2009 | Notes | Contributor(s): Daniel Raftery, Ann Christine Catlin

This document describes the preparation of serum samples in the Raftery Laboratory for metabolomics GCxCG-MS analysis using the LECO GC-TOF mass spectrometer.Serum samples are processed from patient whole blood at the IUSCC clinic immediately after sample acquisition, and then transferred to the …

LECO GC-TOF Instrument Setup: Metabolomics for Cancer Care Engineering

13 Feb. 2009 | Notes | Contributor(s): Daniel Raftery

This document describes the setup for the LECO GC-TOF mass spectrometry instrument in preparation for metabolomics GCxGC-MS analysis of serum samples processed from patient whole blood at the IUSCC clinic and transferred to the Raftery Metabolomics Analysis Laboratory. See also Metabolics GCxGC …

Sample Preparation Protocol: Global Proteomics for Cancer Care Engineering

13 Feb. 2009 | Notes | Contributor(s): Catherine Riley

The urea-based trypsin digestion sample preparation protocol is used for the plasma samples acquired through the Cancer Care Engineering Colorectal Cancer OMIC Pilot Study. Prepared samples are analyzed using the XCT PLUS ESI ion trap located in the Bindley Biosciences Center at Purdue University.

XCT PLUS Instrument Setup: Global Proteomics for Cancer Care Engineering

13 Feb. 2009 | Notes

The document describes the instrument setup for the XCT PLUS ESI ion trap located in the Bindley Biosciences Center at Purdue University. Laboratory analysis for global proteomics research is carried out by Jiri Adamec and Paige Riley. The sample preparation protocol describes the metholodology for …

Data Mining Approach: Global Proteomics for Cancer Care Engineering

14 Feb. 2009 | Notes | Contributor(s): Catherine Riley

The Proteomics Discovery Pipeline (PDP) bioinformatics infrastructure created at the Bindley Bioscience Center (at Purdue University) will be used for data management and data mining.

XCT PLUS: Agilent 1100 Series HPLC-Chip/MS System

14 Feb. 2009 | Notes

Nanospray LC/MS is well established as state-of-the-art technology because of its high sensitivity and low sample consumption. It is most often used for applications with limited sample amounts or when there is a need for analysis of trace level components in complex mixtures. Although nanospray …